Differences between revisions 20 and 22 (spanning 2 versions)
 ⇤ ← Revision 20 as of 2006-12-18 19:33:24 → Size: 1787 Editor: bmb14 Comment: ← Revision 22 as of 2006-12-18 19:43:03 → ⇥ Size: 1915 Editor: bmb14 Comment: Deletions are marked like this. Additions are marked like this. Line 26: Line 26: 1. KLH . 1. '''KLH''' Line 34: Line 35: . ii b converges to a along with the porgress of 3D refinement . ii b converges to model created in a along with the porgress of 3D refinement Line 36: Line 37: . iii The published model could be applied CTF corrections. Hence, we cannot use FRC to compare the similarity between currently reconstructed two models with the published model . iii The published model could be applied CTF corrections. Hence, we cannot use FRC to compare the similarity between currently reconstructed two models with the published model unless we do same correction. (it is difficult, because this requires ctf parameters of particles )

# Requests

### 11/30/06 PAP

Zhong - work on CTF and on high-res refinement

### 12/15/06 PRB

PAP - compose_transform returns negative Eulerian angles sometimes; should always return positive.

• PAP, PRB - how to get angles for a given symmetry group (sample3D)
• how to get boundaries of an asymmetric unit.

PRB from Transform class paper

• sample3D();
• set_rotation(Initial Array, Final Array) ; see paper
• RA.get_sym("c3,2);
• RA.get_sym("icos",3,"orientation")
• RA.get_sym("icos",3, vertex, face);
• RA.get_sym("icos",3, "mrc");
• icos_poss = get_sym_poss("icos")
• Top_face = get_face("icos",3,"mrc")
• em_image.py
• Consolidate some of the rotation calls; Get rid of EMAN calls

PRB regarding Sidewinder

• ratcheting move not working 100% correctly

tests on model created by sidewinder:

. 1. KLH

• a Results in /mnt/shared/prbTestKLH/TEST_1/REFI15 KLH single particle 3D reconstruction, which uses KLH-100-class-averages-refined sidewinder model as the reference model
• b Results in /mnt/shared/prbTestKLH/TEST_2/REFI15 KLH single particle 3D reconstruction, which uses KLH-100-class-averages-refined randomly-assigned-angles model as the reference model

Conclusions

. i 3D volumes in a has better resolutions (FRC) than 3D volumes in b in the initial iterations of 3D reconstructions. a is also more similiar to the published model than b. (high cross correlation value)

. ii b converges to model created in a along with the porgress of 3D refinement

. iii The published model could be applied CTF corrections. Hence, we cannot use FRC to compare the similarity between currently reconstructed two models with the published model unless we do same correction. (it is difficult, because this requires ctf parameters of particles )

. iv Sidewinder gives better initial model than random angle assignement.

Requests (last edited 2017-05-07 11:49:13 by penczek)