Differences between revisions 30 and 31
 ⇤ ← Revision 30 as of 2007-01-02 17:01:48 → Size: 2526 Editor: Philip Baldwin Comment: ← Revision 31 as of 2007-01-04 16:05:36 → ⇥ Size: 2102 Editor: penczek Comment: Deletions are marked like this. Additions are marked like this. Line 2: Line 2: === 11/30/06 PAP ===Zhong - work on CTF and on high-res refinement Line 5: Line 3: === 12/15/06 PRB ===PAP - compose_transform returns negative Eulerian angles sometimes; should always return positive. (fixed December 10th 2006) . PAP, PRB - how to get angles for a given symmetry group (sample3D) . how to get the symmetries in the transform class  . how to get boundaries of an asymmetric unit. Line 23: Line 15: Line 25: Line 18: . Are two sidewinder files necessary (PAP) Line 28: Line 20: === done some 01/03/07 needs more work === Line 32: Line 25: Other: threshhold amplitudes === PRB === Line 44: Line 36: . ii b converges to model created in a along with the porgress of 3D refinement . ii b converges to model created in a along with the progress of 3D refinement

# Requests

PRB from Transform class paper

• sample3D(6,asymmetric_unit",theta_range,phi_range,"method");
• set_rotation(Initial Array, Final Array) ; see paper
• RA.get_sym("c3",2); # This is a rotation element of 120 degrees
• icos_poss = get_sym_poss("icos"); # List the possible conventions of the icosahedron
• Top_face = get_face("icos",3,"mrc"); # represents the vertices of one of faces of the icosahedron
• RA.get_sym("icos",3,"mrc"); # This allows one to access elements of the icosahedron in the mrc convention
• RA.get_sym("icos",3, vertex, face); #This gets the third symmetry elements when the vertex and a face are in specified position
• em_image.py
• Consolidate some of the rotation calls; Get rid of EMAN calls

PRB regarding Sidewinder

• ratcheting move not working 100% correctly

### done some 01/03/07 needs more work

PAP

• Provide better documentation for rotating/shifting an image

### PRB

• tests on model created by sidewinder:

. 1. KLH

• a 3D reconstruction in /mnt/shared/prbTestKLH/TEST_1/REFI15 KLH single particle 3D reconstruction, which uses KLH-100-class-averages-refined sidewinder model as the reference model
• b 3D reconstruction in /mnt/shared/prbTestKLH/TEST_2/REFI15 KLH single particle 3D reconstruction, which uses KLH-100-class-averages-refined randomly-assigned-angles model as the reference model

Conclusions

. i 3D volumes in a has better resolutions (FRC) than 3D volumes in b in the initial iterations of 3D reconstructions. a is also more similiar to the published model than b. (high cross correlation value)

. ii b converges to model created in a along with the progress of 3D refinement

. iii The published model could be applied CTF corrections. Hence, we cannot use FRC to compare the similarity between currently reconstructed two models with the published model unless we do same correction. (it is difficult, because this requires ctf parameters of particles )

. iv Sidewinder gives better initial model than random angle assignement.

Requests (last edited 2017-05-07 11:49:13 by penczek)