Name

recons3d_4nn_ctf - calculate CTF-corrected 3-D reconstruction from a set of projections using three Eulerian angles, two shifts, and CTF settings for each projeciton image

Usage

# calculate reconstruction using odd-numbered projections

Input

stack

name of the stack file containing projection data, projections have to be squares, i.e., nx=ny

list_proj
list of projections to be included in the reconstruction
snr
Signal-to-Noise Ratio of the data (default = 1.0)
sign
sign of the CTF (for convenience for cry data the sign of the CTF is usually inverted and set to +1 so the particles appear to be bright on the dark background) (default = 1)
symmetry
point-group symmetry to be enforced, each projection will enter the reconstruction in all symmetry-related directions. The possibilities are
  • "cn"
  • "dn"
  • "i"
  • default is "c1" (no symmetry).
  • Output

    vol
    the 3-D reconstructed volume

    Description

    The command requires all parameters to be stored in headers of projection images as:

    If projection images were already multiplied by the CTF, set attribute ctf_applied to 1.

    Method

    Reference

    Author / Maintainer

    Pawel A. Penczek

    Keywords

    category 1
    INVERSION
    category 2
    FOURIER

    Files

    reconstruction.py

    See also

    recons3d_4nn, recons3d_sirt

    Bugs

    None. It is perfect.

    recons3d_4nn_ctf (last edited 2015-05-28 20:01:42 by penczek)